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Absorbance

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Jul 24, 2020, 6:28:07 PM
Created by
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Jun 7, 2016, 5:12:37 PM
A=log(P0P)
(P)Irradiance
(P0)Initial irradiance
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088c59af-2cd3-11e6-9770-bc764e2038f2

The absorbance equation calculates the amount of energy  absorbed by a molecule when the molecule is excited by a photon, and is promoted from the ground state (lowest energy level) to an excited state. This is demonstrated in an image below. 

/attachments/088c59af-2cd3-11e6-9770-bc764e2038f2/Absrobance.png

Description

The equation is 

/attachments/088c59af-2cd3-11e6-9770-bc764e2038f2/Screen Shot 2016-06-16 at 6.45.53 PM.png

where

  • P0 is initial irradiance (or intensity of light) from wavelength selector to sample in units of (Energy/sec*area of light beam)
  • P is the irradiance from sample to the light detector in units of (Energy/sec*area of light beam)
  • A is absorbance 

Related Topics

References

Harris, 9th Edition.  Pp.435

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