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Lineweaver-Burk equation

1v0=KMVmax[S]+1Vmax
(Vmax)Reaction Rate with excess substrate
[S]Concentration of Substrate
[KM]Michaelis-Menten Constant

The Lineweaver-Burk equation calculator computes the inverse of the initial velocity of an enzyme inhibited reaction

INSTRUCTIONS: Enter the following:

  • (Vmax) Maximum Velocity of Reaction in moles/(Liter⋅Seconds)
  • [S] Concentration of Substrate
  • [Km] Michaelis-Menten Constant

Inverse Velocity (1/V0): The calculator returns the inverse velocity in seconds-liters per mole (s·L/mol)

The Math / Science

Created by Hans Lineweaver and Dean Burk in 1934, the Lineweaver-Burk equation is a modification of the Michaelis-Menton Equation dealing with enzyme kinetics; allowing it to be represented in graphical-linear form with respect to time. In the equation's markup, KMVmax is the slope of the equation with intercepts of 1Vmax on the vertical axis and -1KM on the horizontal axis.

The Lineweaver-Burk equation is:

  1V0= KmVMAX[S]+1VMAX

where:

  • 1/V0 = Inverse Velocity in seconds-liters per mole (s·L/mol), where v0 is the reaction rate
  • [S] = Concentration of the substrate in moles per liter
  • Vmax = Reaction Rate with excess substrate in units of molsLs
  • KM = The Michaelis-Menten Constant in units of moles per liter