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`A = log((P_0)/(P))`
Enter a value for all fields
The absorbance equation calculates the amount of energy absorbed by a molecule when the molecule is excited by a photon, and is promoted from the ground state (lowest energy level) to an excited state. This is demonstrated in an image below.
Description
The equation is
where
- P0 is initial irradiance (or intensity of light) from wavelength selector to sample in units of (Energy/sec*area of light beam)
- P is the irradiance from sample to the light detector in units of (Energy/sec*area of light beam)
- A is absorbance
Related Topics
References
Harris, 9th Edition. Pp.435
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