Lineweaver-Burk equation

The Lineweaver-Burk equation calculator computes the inverse of the initial velocity of an enzyme inhibited reaction

INSTRUCTIONS: Enter the following:

• (V_max) Maximum Velocity of Reaction in moles/(Liter⋅Seconds)
• [S] Concentration of Substrate
• [Km] Michaelis-Menten Constant

Inverse Velocity (1/V₀): The calculator returns the inverse velocity in seconds-liters per mole (s·L/mol)

The Math / Science

Created by Hans Lineweaver and Dean Burk in 1934, the Lineweaver-Burk equation is a modification of the Michaelis-Menton Equation dealing with enzyme kinetics; allowing it to be represented in graphical-linear form with respect to time. In the equation's markup, `K_M/V_max` is the slope of the equation with intercepts of `1/V_"max"` on the vertical axis and `-1/K_M` on the horizontal axis.

The Lineweaver-Burk equation is:

  `1/V_0 = K_m/(V_(MAX) * [S]) + 1/V_(MAX)`

where:

• 1/V₀ = Inverse Velocity in seconds-liters per mole (s·L/mol), where `v_0` is the reaction rate
• [S] = Concentration of the substrate in moles per liter
• V_max = Reaction Rate with excess substrate in units of `"mols"/(L*s)`
• K_{M =} The Michaelis-Menten Constant in units of moles per liter